Hi..
can anyone tell me what is the method for virus inactivation so that it can be used for immunization in animals, especially in mice.
Dear Manpreet Kaur
Several virological and immunological assays are used for the characterization of a virus and the immune response induced
Pls. find the attached files, may help in your research, good luck
https://www.aphis.usda.gov/animal_health/vet_biologics/publications/308.pdf
https://www.hindawi.com/journals/av/2015/616898/
http://www.paho.org/hq/index.php?option=com_docman&task=doc_view&gid=27982&Itemid=270
http://www.who.int/bloodproducts/publications/WHO_TRS_924_A4.pdf
http://www.jimmunol.org/content/172/9/5598.full.pdf
http://www.microbiologyresearch.org/docserver/fulltext/jgv/91/6/1450.pdf?expires=1472586200&id=id&accname=guest&checksum=D3F629BE20849952BBDB4571322B6F4B
http://citeseerx.ist.psu.edu/viewdoc/download?doi=10.1.1.585.3863&rep=rep1&type=pdf
http://onlinelibrary.wiley.com/doi/10.1111/irv.12150/pdf
http://jid.oxfordjournals.org/content/180/3/579.full
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782010001100013
http://www.sciencedirect.com/science/article/pii/S0042682205001418
http://jcm.asm.org/content/48/3/928.full
http://www.nature.com/icb/journal/v78/n2/full/icb200021a.html
http://www.biondvax.com/wp-content/uploads/2015/06/19-efficacy-of-anti-influenza.pdf
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC393254/pdf/pnas00023-0341.pdf
http://jid.oxfordjournals.org/content/180/3/579.short
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3697442/
https://www.miltenyibiotec.com/~/media/Files/Navigation/Sample%20preparation/Customer%20protocols/Isolation%20of%20Influenza%20A%20from%20mouse%20lung.ashx
http://journals.plos.org/plosntds/article?id=10.1371%2Fjournal.pntd.0004167 https://virologyj.biomedcentral.com/articles/10.1186/1743-422X-8-251
https://www.researchgate.net/profile/Peter_Liljestroem/publication/11397352_Palmowski_M._J._et_al._Competition_between_CTL_narrows_the_immune_response_induced_by_prime-boost_vaccination_protocols._J._Immunol._168_4391-4398/links/0046351d13ce007de0000000.pdf
http://www.cdc.gov/mmwr/pdf/rr/rr4201.pdf
https://www.cdc.gov/mmwr/preview/mmwrhtml/00020599.htm
http://jvi.asm.org/content/78/18/9624.full
Regards
Prof. Houda Kawas
Article Competition Between CTL Narrows the Immune Response Induced ...
Dear Houda and Manpreet - thanks for your interesting discussion on virus inactivation for mouse studies, and for your interest in our 2000 ICB article on oral influenza vaccination.
Using different doses of gamma-irradition (13 - 30 kGy) to inactivate A/Qld influenza was fascinating in terms of the subsequent post-challenge antibody response (presumably related to partial of full virus inactivation prior to oral vaccination). We described this also in an earlier Vaccine publication (Lidbury B.A., et al. (1997). Erythrocytes enhance the immunogenicity of oral vaccination with gamma irradiated influenza virus: increasing the dose of irradiation results in a significant diminution of lung IgA response. Vaccine 15:1529-1537), which provides additional insights on the vaccine preparation.
Best wishes, Brett Lidbury.
Try Beta propiolactone to 1/4000 . If you are working with small amounts , increase the inactivating dose. It is useful in such cases to dilute the GLP in sterile distilled water. Allow the mixture gently stirred for 2 hours at laboratory temperature and then overnight at 4 ° C . Intraperitoneally immunization of mice with recall 7 days after can give you satisfactory, I hope.
Good continiation
We have been using a hydrogen peroxide based platform. See Ammanna, Raue & Slifka Nat Med 2012, Walker, Raue & Slifka JV 2012, Pinto et al. JV 2013. Beta propiolactone & Formaldehyde are the traditional methods, but we have found that these while efficacious have 'problems'. The most significant one being the 'trailing' in the kinetics of inactivation when using formaldehyde necessitating long inactivation times and/or elevated temperatures, and the (potential) epitope damage when using either chemical.