21 April 2023 1 9K Report

What is the reason that viruses aggregate in aqueous solution ??

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How to use SILVA to find Eukaryotes cell HEK-293T cells 18S RNA sequence ?

How to use this website to search 18S RNA sequence for HEK-293T cells specifically?? Thanks

30 July 2023 9,723 0 View

What is on the membrane ( SEM image) ?

I filtered sodium dodecyl sulfate in buffer solutions through a PES membrane. After that, I took some SEM images of my sample to visualize organic particles fouling on membrane. Here is one of my...

21 July 2023 7,908 4 View

Why my AdV particles are elongated?? (ATCC VR5) ?

Question about TEM images of VR-5 (Adenovirus A05) I attached my virus TEM images. I am confident that the particles in the image are adenovirus because the size of the particles is similar to...

16 July 2023 3,434 5 View

Why my TEM image shows a positive staining even though i use uranyl acetate to negatively stain the sample??

Here is an image I tried to take using TEM ( JEM-1400S) for human adenovirus. I used uranyl acetate to negatively stain the sample. However, this image I have indicates the sample is positively...

27 June 2023 461 1 View

What should I use between primer and probe in qPCR?

I'm trying to design the qPCR method. If I already have two primers and a probe sequence, how am I going to connect the primer and probe sequence together? ( see picture) Can I use, for example,...

13 June 2023 3,893 2 View

SEM pictures of the same membrane look different. Why??

I took SEM pictures of my clean 0.2um pore size PES membrane filter. The first picture is coated with 5nm gold, and the second is with 20 nm carbon. Both pictures are 2500X magnification, and the...

13 March 2023 4,830 24 View

Why All protein IEP increase between PH 6 to PH8 ?

I did an IEP modeling curve for a virus surface protein (human adenovirus A-05. I wonder why the surface protein PI will increase between PH 8 and 10. Do u guys have some recommended reference...

05 March 2023 4,199 0 View

How to know if a mammalian cell is dead?

How to check if a mammalian cell is dead? can I use a dye? How to regrow the cell to check the survivability?

10 July 2022 289 5 View

Is my L929 (NCTC 929) cell line look normal ?

Hello, this is the third day that I grow L-929 cell line. Since yesterday, the cells aggregate and become like this big cluster. I want to know if this is normal. How to break the aggregation??...

02 June 2022 6,438 0 View

How Sodium dodecyl sulfate (surfactant) affect cell membrane ?

Hello, I'm wondering how SDS (surfactant) interact with cell membrane? the mechanism behind it. I heard it is used to denatured proteins. I want to know which protein residue does it affect...

15 May 2022 9,645 1 View

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It is being seen that Editors of some reputed Journals are putting on hold the manuscripts and not updating review states even passing more than six months from submission. Researchers are...

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Our lab has some radioactive cell samples that need to be kept frozen for at least 30 days before flow analysis. For some reason, the samples stored in liquid nitrogen did not perform as well as...

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