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Questions related from Makayla kiki Loey
How to use this website to search 18S RNA sequence for HEK-293T cells specifically?? Thanks
31 July 2023 9,695 0 View
I filtered sodium dodecyl sulfate in buffer solutions through a PES membrane. After that, I took some SEM images of my sample to visualize organic particles fouling on membrane. Here is one of my...
22 July 2023 7,874 4 View
Question about TEM images of VR-5 (Adenovirus A05) I attached my virus TEM images. I am confident that the particles in the image are adenovirus because the size of the particles is similar to...
17 July 2023 3,406 5 View
Here is an image I tried to take using TEM ( JEM-1400S) for human adenovirus. I used uranyl acetate to negatively stain the sample. However, this image I have indicates the sample is positively...
28 June 2023 433 1 View
I'm trying to design the qPCR method. If I already have two primers and a probe sequence, how am I going to connect the primer and probe sequence together? ( see picture) Can I use, for example,...
14 June 2023 3,853 2 View
What is the reason that viruses aggregate in aqueous solution ??
21 April 2023 8,601 1 View
I took SEM pictures of my clean 0.2um pore size PES membrane filter. The first picture is coated with 5nm gold, and the second is with 20 nm carbon. Both pictures are 2500X magnification, and the...
14 March 2023 4,804 24 View
I did an IEP modeling curve for a virus surface protein (human adenovirus A-05. I wonder why the surface protein PI will increase between PH 8 and 10. Do u guys have some recommended reference...
06 March 2023 4,167 0 View
How to check if a mammalian cell is dead? can I use a dye? How to regrow the cell to check the survivability?
11 July 2022 264 5 View
Hello, this is the third day that I grow L-929 cell line. Since yesterday, the cells aggregate and become like this big cluster. I want to know if this is normal. How to break the aggregation??...
03 June 2022 6,412 0 View
Hello, I'm wondering how SDS (surfactant) interact with cell membrane? the mechanism behind it. I heard it is used to denatured proteins. I want to know which protein residue does it affect...
16 May 2022 9,620 1 View
does anyone has NCTC 929 cell line ? I need the protocol for this cell line. where do u usually find a cell protocol? thanks
04 May 2022 1,120 1 View
I'm a second year PhD student. If I want to do a post-doc after my PhD, when should I start looking for a school and contact PI?
01 January 1970 1,376 1 View
