How to use this website to search 18S RNA sequence for HEK-293T cells specifically??
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I filtered sodium dodecyl sulfate in buffer solutions through a PES membrane. After that, I took some SEM images of my sample to visualize organic particles fouling on membrane. Here is one of my...
21 July 2023 7,908 4 View
Question about TEM images of VR-5 (Adenovirus A05) I attached my virus TEM images. I am confident that the particles in the image are adenovirus because the size of the particles is similar to...
16 July 2023 3,434 5 View
Here is an image I tried to take using TEM ( JEM-1400S) for human adenovirus. I used uranyl acetate to negatively stain the sample. However, this image I have indicates the sample is positively...
27 June 2023 461 1 View
I'm trying to design the qPCR method. If I already have two primers and a probe sequence, how am I going to connect the primer and probe sequence together? ( see picture) Can I use, for example,...
13 June 2023 3,893 2 View
What is the reason that viruses aggregate in aqueous solution ??
20 April 2023 8,625 1 View
I took SEM pictures of my clean 0.2um pore size PES membrane filter. The first picture is coated with 5nm gold, and the second is with 20 nm carbon. Both pictures are 2500X magnification, and the...
13 March 2023 4,830 24 View
I did an IEP modeling curve for a virus surface protein (human adenovirus A-05. I wonder why the surface protein PI will increase between PH 8 and 10. Do u guys have some recommended reference...
05 March 2023 4,199 0 View
How to check if a mammalian cell is dead? can I use a dye? How to regrow the cell to check the survivability?
10 July 2022 289 5 View
Hello, this is the third day that I grow L-929 cell line. Since yesterday, the cells aggregate and become like this big cluster. I want to know if this is normal. How to break the aggregation??...
02 June 2022 6,438 0 View
Hello, I'm wondering how SDS (surfactant) interact with cell membrane? the mechanism behind it. I heard it is used to denatured proteins. I want to know which protein residue does it affect...
15 May 2022 9,645 1 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
Is this website real? https://isar.org.in/event/registration.php?id=2434532
08 August 2024 484 1 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
A website software of Blackbody radiation law expert software can used through the following web site. http://39.105.188.151:3000/index
07 August 2024 1,706 0 View
I have face this problem anyone help me how to solve this issue ?which is below Fatal error: There are inconsistent shifts over periodic boundaries in a molecule type consisting of 78 atoms. The...
07 August 2024 2,598 1 View
Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...
06 August 2024 807 3 View