Vector Information.....?

More Manish Joshi's questions See All

I have some queries about basics of neuroscience...?

1. There is much greater concentration of K+ ions inside the cell than outside, why then is the resting membrane potential always negative? 2. If you poison a neuron with a metabolic inhibitor...

09 October 2015 4,314 2 View

How can CoCl2 mimic hypoxia?

Hello, I am curious to know the biochemical reactions of CoCl2 that mimic hypoxia. Is it that CoCl2 can bind any ubiquitin enzyme which regulates their degradation?

11 December 2014 903 4 View

Why am I getting a nonspecific HIF-1alpha blot?

Hello, I am getting nonspecific bands in HIF-1alpha blot. Can any one suggest why and how I can improve this? I am using the following conditions Cell: HCT116 Gel: 8% Blocking: 5% non fat milk...

10 November 2014 4,956 5 View

How do you induce Hypoxia in cancer cell lines?

Hi, I want to induced hypoxia in cancer cell lines to check regulation of VEGF signaling components. So is there any simple way to induced hypoxia in breast cancer cell lines like HCT116, MCF7 or...

09 October 2014 4,860 7 View

How do you protect proteins from precipitation and degradation?

Hi, I am purifying proteins for crystallization, but sometimes they precipitate and degrade. What are the tips and precautions I should take during purification and storage? Is there any...

09 October 2014 777 14 View

How to identify Drosophila Mutant...?

I am searching literature about Drosophila mutant identification. can anyone suggest me books or papers related to Drosophila mutant identification and their maintenance...? I also need previous...

08 September 2014 8,253 4 View

How do you check Sensitivity of PCR ?

Hello, I want to know that how to check sensitivity of PCR ?

08 September 2014 313 8 View

Why are tumor suppressor proteins down regulated in cancer cells?

Why are tumor suppressor proteins down regulated in cancer cells even they are not mutated. Can some one explain signaling for this down regulation of tumor suppressor protein with any example or...

08 September 2014 4,881 4 View

Can anyone provide me a protocol for Fusarium oxysporum culture?

I want to culture Fusarium oxysporum. Can anyone provide me detailed protocol for Fusarium oxysporum culture?

02 March 2014 10,128 13 View

Does quantum mechanics fit to explain such brain functions as behaviour, neurodegeneration and consciousness?

Or is brain function a biological phenomenon? My other question is whether consciousness really exists in brain or is it a product of brain activity?

01 February 2014 8,805 46 View

If we are using snowball sampling technique, how do we justify the true representativeness of the sample statistically? is there any statistical test?

Are there any statistical methods to justify your sampling technique using SPSS or AMOS?

05 August 2024 9,153 4 View

What are possible strategies can be used to analyze data under sequential explanatory mixed method approach?

Better ways to analyze the qualitative and quantitative data in a sequential explanatory mixed method approaches

04 August 2024 2,703 6 View

Why 3 replicates for most biological assays? Is it enough to examine the data fits normal distribution?

Just bounced on me. Before statistically analysing significant difference, shouldn't we see if data fits normal distribution first? Is 3 replicates enough to testify the hypothesis of normal...

31 July 2024 8,141 13 View

Request for Advice: Starch Metabolism Research Project?

I am currently considering a research project focusing on a comparative analysis of starch metabolism in orchids and roses. I am particularly interested in identifying the types and quantities of...

30 July 2024 4,267 2 View

Can the limit of quantification (LOQ) of an analytical method fall outside its linear dynamic range, or must it always be within it?

Can an analytical method's limit of quantification (LOQ) be outside its linear dynamic range, or is it always required to be within it? Please provide a thorough explanation supported by verified...

29 July 2024 7,198 9 View

Pragmatic inquiry research design?

Employing a pragmatic inquiry research design, looking for published research using this method, employing qualitative research data collection methods of semi-structured interview and focus...

28 July 2024 540 2 View

Can i apply a public questionnaire but analyse it with a different data analysis method?

First, thanks for taking the time to read my puzzle 🥹! I am currently working on the methodology part of my dissertation, and I have chosen a questionnaire as the instrument for my quantitative...

27 July 2024 3,457 3 View

How to determine method detection limit in an analytical method?

I know the difference between instrumental LOD and method LOD but my query is - in case of any sample whose concentration is zero or not detected by the instrumental LOD, is it possible to get...

24 July 2024 6,592 5 View

Do we put respondents data in research methods or findings section of a qualitative study?

Are both ways acceptable, or is there any convention to them? Thanks.

22 July 2024 6,988 4 View

Restaurant research, methods of quantitative and qualitative statistics?

Greetings! I am looking for materials on quantitative and qualitative research. What are the methods of collecting information and the principles of their implementation? I would like to make an...

20 July 2024 2,033 7 View

Patrycja Koszałka

Try Addgene: http://www.addgene.org/

It is a great place to look for the information about the plasmid vectors.

Surender Khatodia

You can check this out also

http://www.cambia.org/daisy/cambia/585.html

pCambia Vectors

Widely used plasmid vectors from CAMBIA

Manish Joshi

thank u Patrycja and Surender for suggesting me places for vector informations