Apart from the Ramachandran plot, what are the various ways of validating the structure of a modeled protein? What are the parameters one need to consider when validating a modeled protein structure.
Errat, Verify3D, Procheck. If you have the template structure you can check the superimpose RMSD by using the PyMol.
This is Interesting
Hi, what should we do if the predicted structure for our protein design does not pass the PROCHECK assessment?
I recently received this email message: "Dear Colleague, I trust you are well! I am writing to propose a mutually beneficial collaboration. I have many research papers that need citations. If...
27 June 2024 9,627 1 View
Looking for a protocol for the stimulation of B cells with pokeween mitogen
22 June 2024 4,882 4 View
Frameworks for AI governance The use of artificial intelligence in the company requires its control and monitoring by the company's governing bodies due to legal and regulatory requirements....
17 June 2024 8,956 5 View
can someone use quantum mechanics to explain the intuition behind this phenomenon?
07 June 2024 9,584 2 View
Elderly patients may develop strokes associated with high blood pressure. Small strokes may result in cognitive dysfunction, confusion, memory lapses and onset of dementia. less frequently and...
07 June 2024 8,723 2 View
to "Familiar biological, chemical and physical events credibly evolve the Standard Genetic Code", for example?
06 June 2024 5,095 1 View
Cytosine methylation, histone acetylation, etc. represent distinct epigenetic mechanisms of gene regulation. Which example is most likely to account for transgenerational effects, such as...
31 May 2024 4,613 3 View
I'm trying to stain areas of dystrophic calcification in sections of FFPE perinatal brain tissue. Typical basophilic appearance on H&E but nothing with Alizarin Red (costochondral junction...
28 May 2024 4,068 2 View
I am working on vehicle pedestrian safety at 4-way intersections with traffic signal control lights. I need dataset available for Kaduna metropolis busy 4-way intersection with pedestrian count...
21 May 2024 4,872 1 View
Where can I find Business and Professional Communication Quarterly's manuscript acceptance rate?
18 May 2024 1,303 1 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....
29 July 2024 5,007 0 View
I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...
28 July 2024 366 3 View
I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...
28 July 2024 3,178 3 View
It has been long known that EDC carbodiimide and NHS easter's half life agains hydrolysis are highly related to pH. However, very surprisingly, not yet found a paper giving a table/chart of their...
25 July 2024 8,738 0 View
In running two-dimensional gel electrophoresis on bacterial protein, some spots that appear to match a protein sequence have a significantly more acidic isoelectric point than the calculated pI....
24 July 2024 8,076 3 View
I found that several expression constructs of published protein structures containing an N-teiminal fusion tag GAMGSGIQRPTST. What's the founction of this fusion tag?
23 July 2024 1,353 4 View
I had a problem for so long. I could easily delete elements in ABAQUS implicit solver but I needed to use VUMAT so I had to have Explicit solution but the element did not disappear for so long. I...
23 July 2024 10,032 0 View
hi every one I am making vector construction (for fusion proteins) and in this moment I wanna to amplification of ADAM17 prodomain with PCR. to yet, I couldn't amplified the ADAM17 prodomain with...
21 July 2024 8,660 1 View