By itself UV/VIS is much less specific than IR, NMR, MS, LC, GC/MS and other techniques. It can be used, however, very well for quantitative analysis if the composition and the possible impuriites are known from other methods. It can be, however, very useful in pH dependent studies, in association studies, in solvatatiton studies, in temperature dependent and kinetic studies.
Doctor Gyorgy Banhegyi thank you very much for your contribution. The method is specific? the Western European Laboratory Accreditation Conference (WELAC ) say that only a method which is perfectly selective for an analyte or group of analytes is said to be specific. But this method (UV/VIS) it is not.
Well, in science "perfect" is not an appropriate attrribute. We can talk about the degree of selectivity or specificity. Those methods which provide more details (as IR, NMR or MS) are naturally more specific - especially if not a single band, but a set of band is used for identification. One should alos distinguishe between qualitative and quantitative analysis. UV-VIS is excellent for quantitative analysis, but less "perfect" for qualitative analysis. The reason is that the UV-VIS bands are fairly broad and their number is relatively small. If any other component absorbs in the UV-VIS range, it is not easy to separate the effects.
If you apply derivative spectroscopy (transforming the obtained spectra to the 4th derivative) you can gain much more specific results.
Dear Andrei V. Pipa, Ultraviolet spectrophotometry, UV Equipment
I agree above explaining reasoning, But my answer in term specific which is more reliable method validation in favour of UV-VIS spectrophotometry,Again selectivity concern ,those type of compound containg chromophore, auxochrome, polyene conjugation, (such as conjugated chromophore and aromatic molecule) are uv sensitive generally more preferable.Although geometric correction, derivative spectroscopy & subject the drug to an operation ( chemical reaction, change of solvent) are sometime measure the drug concentration within extreme used in the Beer's law study. SIngle component & multicomponent analysis in assay context high sensitivity.
In means of solid sample the intensity of an absorption band basically probable for the transition. The theoretical prediction of absorption intensity is not practical, but an approximate value may be estimated by analogy with spectral data for closely related component.
UV- VIS Spectrophotometery is selective at specific wave length for a particular parameter using Standard Method APHA.
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