Hello,
I am planning to start a project on UVB induced skin cancer. For that I read articles and found the different methods used by the researchers for UVB, but I am unable to identify the design of the apparatus. It will be highly appreciable, if someone can guide me to the design of the chamber.
The following papers may be useful. For cultured cells, cells need to be washed twice or thrice with PBS prior to irradiation. For animals, shaved areas to be exposed to UV need to be shaved (e.g., with electric clippers) prior to irradiation, or hairless animals are used.
https://www.ncbi.nlm.nih.gov/pubmed/25925725
https://www.ncbi.nlm.nih.gov/pubmed/26869824
https://www.ncbi.nlm.nih.gov/pubmed/28587261
https://www.ncbi.nlm.nih.gov/pubmed/28558005
https://www.ncbi.nlm.nih.gov/pubmed/28548949
Dear Shah,
You may choose Wistar albino rats for the ultraviolet ray B-induced photodermatitis. For induction, shave 10% dorsum area (1.5 × 2.5 cm2) of rat skin using depilatory cream/ hair clipper. Irradiate the shaved portion of skin for 15 min (1.5 J/cm2) at a vertical distance of 20 cm with UV-B lamp.
There are several literatures available describing the UV-B range of 280-320 nm with the exposure time in the range of 15-30 min for the induction of psoriasis. You may observe variability in the UV-B range and exposure time for the induction of psoriasis. We have found psoriasis-like symptoms at a range of 385 nm for 30-45 min. After irradiation for 30 min, we observed the redness on rat’s skin followed by the gradually increasing erythema after 6-8 h. After 48 h there was the presence of silvery red scale scales on exposed rat’s skin.
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