By mistake I kept some for my flower bud samples in paraformaldehyde for more than a week (usual time if few hours), can i still wash them with 1x PBS and store them in 70% ethanol at 4 C ?
(Currently they are in 4% paraformaldehyde @ 4C from past 9 days)
Anyone went through the same problem ?
Dharmesh Jain
Thanks for the reply.
I tried using those samples, I work on pollen and samples were flower buds. After the paraformaldehyde treatment, I was unable to take out the pollen from the anthers.
When I tried applying more pressure, I ended up crushing all the pollen and bringing out other anther wall layer (Tapetum) out.
Any suggestion on that ?
0 votes
0 thanks
- Badges
- Science topic
- Similar topics
- Biological Science
- Biota
- Eukaryota
- Viridiplantae
- Streptophyta
- Plants
More Dharmesh Jain's questions See All
Similar questions and discussions