please if anyone has used IL-6 in treatment of prostate cancer cell line research , i want to ask about the protocol
Cells were treated with 100 ng/mL of IL-6 in serum free media for 1 hr, then lysed. (Pancreas. 2012 Jul; 41(5): 773–781. IL-6 stimulates STAT3 and Pim-1 kinase in pancreatic cancer cell lines)
For IL-6 treatments semi confluent cells were rinsed in phosphate-buffered saline (PBS) and incubated for 2, 5, 10 or 20 h with 20 ng/ml or 40 ng/ml IL-6 diluted in DMEM (Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchVolume 1783, Issue 10, October 2008, IL-6 induces MUC4 expression through gp130/STAT3 pathway in gastric cancer cell lines).
Article IL-6 induces MUC4 expression through Gp130/STAT3 pathway in ...
Article IL-6 stimulates STAT3 and Pim-1 kinase in pancreatic cancer cell lines
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