Cells were treated with 100 ng/mL of IL-6 in serum free media for 1 hr, then lysed. (Pancreas. 2012 Jul; 41(5): 773–781. IL-6 stimulates STAT3 and Pim-1 kinase in pancreatic cancer cell lines)

For IL-6 treatments semi confluent cells were rinsed in phosphate-buffered saline (PBS) and incubated for 2, 5, 10 or 20 h with 20 ng/ml or 40 ng/ml IL-6 diluted in DMEM (Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchVolume 1783, Issue 10, October 2008, IL-6 induces MUC4 expression through gp130/STAT3 pathway in gastric cancer cell lines).

Article IL-6 induces MUC4 expression through Gp130/STAT3 pathway in ...

Article IL-6 stimulates STAT3 and Pim-1 kinase in pancreatic cancer cell lines