I was wandering if these kind of mice are good only for studying cancer or they can be used for Alzheimer disease. Do you have any suggestions or experience with this model?
title topic " Role of Facility Layout in enhancing workplace safety and ergonomics in Batangas State University"
06 May 2024 8,388 1 View
Hi, I observed a strange behaviour in my cell lines I had never seen before. Those are stable lines from Ovarian cancer (line's name: HEY) and from Prostate cancer (line's name: PC-3). Both lines...
08 April 2024 1,418 0 View
Dear friends, I am Stefano Cipriani and dong an ecolabel that will be useful for the market, especially to increase use of recycled materials. I have a question that would love to share here in...
27 March 2024 5,298 3 View
Consider two clocks set just under the surface of water, they are synchronized. One of them departs at velocity v and then rejoins at -v. The overall time dilation must be the same as the one in...
14 March 2024 2,203 86 View
I recently discovered that BFA is used in flow cytometry, enabling the observation of intracellular cytokines that would be otherwise released. Is the same principle applicable to ROS? I would...
04 March 2024 7,094 1 View
Does an ip E.coli injection (10^8 ) cause death in 4 hours in C57BL/6? Do I need to hydrate the mouse? Is this a good concentration to mimic a septic situation?
04 March 2024 4,552 1 View
I'm searching for a comprehensive textbook or paper on power-split and compound architectures in hybrid electric vehicles (HEVs). I've come across some very interesting papers on HEVs. However,...
11 February 2024 8,516 0 View
Good day, may you suggest a reference method for micrometers calibration? Thanks in advance Stefano
06 February 2024 8,027 3 View
Hi ResearchGate community, can anyone help us out with this contamination? We work with iPSC-NPC. This contamination happens ONLY when we put the neuronal progenitors in coverslips + media for...
14 December 2023 6,435 0 View
Dear all, do you know what is the correct subsample size (number of pixel) to carry out a confusion matrix? I am digitizing the true classes on an image to check the accuracy of the classifier....
11 August 2023 6,528 2 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
I received an e-mail invitation to join the editorial board of Clinical Cardiology Updates. While I have published a few articles related to cardiovascular disease, there are lots of colleagues...
06 August 2024 8,981 8 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have a theoretical framework for preparing a interview guide to assess the unmet care needs of a sub population of chronically ill patients and their caregivers(dyadic interviews).
04 August 2024 8,798 7 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View
I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...
31 July 2024 9,186 0 View
I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...
31 July 2024 1,123 4 View