Is there any chemical or surgical method to induce hypoxia in mouse model?

Dear all, My question is about Inducible hypoxia in vivo (mouse model). I'm looking for some chemicals that can induce the pathway of hypoxia or some other techniques. If you now know something...

15 December 2020 866 6 View

Acetic acid in liquid waste ?

Hy, may you suggest a method to quantify acetic acid in wastes or leaching test? Thanks in advance Stefano

10 December 2020 4,023 2 View

Which points I have to take in mind to mutate a plasmid that grow in Neb stable strain?

Several kits that allow the introduction of point mutations in plasmids say to transform mutated plasmids in DH5alpha bacteria. I have a plasmid with many repetitions that need stable strains to...

08 November 2020 4,250 2 View

Did you have any problem for staining nuclear phosphorylated proteins in hippocampal neurones?

I was wondering if there may be a problem of permeabilisation of cells. I am working on mice hippocampus (pups) and I need to make a staining for a phoshoprilated nuclear protein. Since the...

17 September 2020 2,000 3 View

Glass or PVC material to perform a soil leaching experiment of pharmaceuticals?

Hello I am developing a soil column experiment to study pharmaceuticals transport and attenuation.I would like to know If is better to use a glass or a PVC column to perform this experiment and...

10 August 2020 6,214 3 View

How many researchers are interested in attending web communications in the field of Sustainable Planning?

17 June 2020 4,753 74 View

What happens to non-"preclincial essential research" (non Covid19) in Covid times?

Are you askiing what researchers are doing in Covid time especially those that work with animal? I started a survey to collect information (anonymous) to see whether there are alternatives to...

02 April 2020 9,596 3 View

Generic DC/HYBRID grid-connected microgrid available in simulink?

I'm looking for a microgrid simulation model written in simulink in order to implement my simulation data and test my Energy Management System algorithm. The microgrid I am looking forward is a...

24 October 2019 3,541 4 View

Is there an investigation about the real world implementation of decision making algorithms in smart home/microgrids EMS microcontrollers?

Implementation of stochastic and probabilistic based algorithm is a very tricky task especially in energy management systems microcontrollers. I'm looking for a research paper which talks about...

30 July 2019 5,947 1 View

What is the best choice for a ribosomal protein used as a loading control in WB?

Hi everyone. I have to assess protein expression in my adult satellite muscle cells by WB. I'd prefer not to use the conventional beta-actin or alpha-tubulin, nor GAPDH. I saw that 18S mRNA was...

24 June 2019 3,133 2 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

Is it harmful to breed for Cre Homozygosity initially?

Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...

24 February 2021 5,759 3 View

How to empty large intestine in mice ?

Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...

23 February 2021 7,701 2 View

Does PHA-P stimulate the proliferation of lymphocytes from mice?

Hello, I will start performing MLR using lymphocytes from mice's spleen and I would like to know the better way for the stimulation. If is using PHA-P or CD3/CD28? I would like to know as well if...

23 February 2021 5,476 4 View

How to culture mouse pheochromocytoma cells (MPC)?

Hi Everyone, Has anyone here worked with MPC cell line? Any tips or tricks? I am just venturing into cell biology and any advice on this topic would be highly appreciated. Thank you so much. Niusha

22 February 2021 3,891 2 View