Hi all,
I just started to prep mouse cortical neutrons for E16.5 and in my second prep I had a massive fungal contamination. Since these preps are quite precious, I wonder if I could add an antimicotic to my supplemented Neurobasal medium to preven contaminations in my next cultures.
Any personal experience or suggestion will be much appreciated.
All the best,
Ernest
Puromycin may help, its been shown to inhibit yeast growth. It has not negatively affected our cultures.
Personally, I decreased contamination in my cultures by improving aseptic technique and treating the incubator water bath with Copper 10g and EDTA 0.2g in 10L H2O. That cut way down on the frequency of culture contamination.
We also grow primaries in covered image chambers stored in poly trays with lids. Both of those are UV'd for at least an hour before getting any substrate.