Users of PPAR Transcription Factor Assay Kit (Abcam)?

23 November 2017 1 2K Report

Has anyone of you used PPAR Transcription Factor Assay Kit (Abcam ab133113)? How many microgrames of protein did you put into the well to detect PPAR gamma? I put up to 50 microgrames and still the signal is still weak...

Xiujing Feng

You can try the antibody from CST, we used

CST - PPARγ (81B8) Rabbit mAb (1:1000) to test 100 microgrames protein.

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