Hi Alex Nguyen

This is a fair point, and I agree that it is worth considering.

The first cell lines to be generated were often from cancerous tissues, as the ability to expand and divide indefinitely is a useful characteristic (both from the cancer, and the researcher's point of view).

The use of (cancerous) cell lines however has become widely accepted, and most people will not discuss this in their research.

An alternative to using cell lines is to use primary cells. The problem here however is that they are more difficult to get (depending on the tissue type of course), are generally more finicky to grow, can show significant heterogeneity, and are less well characterised.

In short, I agree with your opinion and believe it should be discussed more widely, but reviewers are unlikely to make a fuss if you don't mention it in your discussion.

Sam

If your problem is that they don't grow in your system, HPEG2 us human cell line, won't grow in immunocompetent animal model. you should use a RAT cancer cell line for your experiments. or you need an immunodeficient host to have human tumor growth in animal moel.

good luck

Michela