I'm doing RNA extraction with TRIZOL, but I just ran out. I saw some bottles identical to the TRIZOL so I assumed I had more but it turned out to be "buffer saturated phenol".
is this the same as TRIZOL? Can I use buffer saturated phenol with the same volume as I use TRIZOL?
If it isn't the same, is it possible to freeze the cell pellet so that I can do RNA ext when I can get more TRIZOL?
Thanks!
Yes u can use the buffer saturatd phenol. Please do check the pH of the solution. it should be 4-6 . and also mention which buffer preferably TRIS buffer is used.
Trizol is a mixture of phenol, chloroform, and guanidinium isothiocyanate, so it isn't the same thing as buffer-saturated phenol.
Another way, add 1% SDS and continue deproteinization according to the standard protocol (see Maniatis, Short Protocols Mol.Biol., etc). Usually, as a result, you have RNA+DNA. You can separate RNA by precipitation with 2 M LiCl or treat a sample with DNase and remove it repeating deproteinization. Pure RNA can be precipitated with LiCl or alcohol.
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