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Questions related from Nathaniel Zhu
I'm trying to remove dead cells from human Mononuclear cell culture without either slow centrifugation or ficoll. I would like to remove dead cells by pulvevrising them into debris (smaller than...
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I have an old FACScan that I'm trying to get working. It connects to cellquest fine. Drain, fill, standby, and backflush also works fine. Laser works fine. Dongle works fine. BDPac shows...
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I'm trying to extract the White Blood cells from Blood for cell culture. I'm currently using LSM to get the mononuclear cells but I think it would be easier just to lyse the red cells directly...
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I'm doing RNA extraction with TRIZOL, but I just ran out. I saw some bottles identical to the TRIZOL so I assumed I had more but it turned out to be "buffer saturated phenol". is this the same as...
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We are trying to detect and quantify the presence of KLF2 which should appear as we have memory cells (CCR7+/CD62L+). We're using western blotting for that and we've tried both the traditional...
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