Urethane doses - sex and strain differences?

28 February 2019 0 8K Report

If you use urethane for anesthesia purposes in mice, can you share differences you have noticed between sexes and strains? We are noticing females are more sensitive (C57 to some degree, but majorly so in DBA2 mice). Similar experiences?

Badges
Science topic

Similar topics
Eutheria
Rodentia
Muridae
Murinae
Mice

More Deranda Lester's questions See All

I sent on five new publications( 2 months ago) but have not seen them?

I sent on five new publications but have not seen under my name Dr. Lester Mandelker

10 June 2020 7,206 3 View

To create a Chi-map, do I need to calculate an mn ratio per stream or can I use a general value per watershed?

Hello I'm currently trying to create a chi-map using topotoolbox for matlab. In the available literature most of the calculations use a single mn ratio (0.45-0.5) for the entire area, and some...

17 February 2019 6,964 4 View

About center of gravity of an object (centroid)?

The center of gravity of an object(centroid) is the same if the object is represented by shape or contourn?

29 April 2016 334 10 View

How to induce in images blur simulating out-of-focus optics in matlab or octave?

I want induce in images different kind of blur like: -Movement during the image capture process, by the camera or, when long exposure times are used, by the subject (Linear Blur) -Out-of-focus...

05 November 2015 4,885 3 View

Looking for mitragynine and 7-hydroxymitragynine (extracted and synthesized from Kratom)

Does anyone have any advice on buying (or collaborating with someone willing to share) compounds extracted and synthesized from Mitragyna speciosa (kratom)? I'm specifically looking for...

01 January 1970 785 1 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

How to compare 2 samples recruited with different sampling methods?

If I want to compare characteristics of 2 groups of patients, one group is recruited from a random sample of the general public while another group is from a sample with a fixed gender (male to...

26 February 2021 3,526 3 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

Experimental Design: How deal with pseudreplicates with immunological and clinical-chemical paramaeters?

Hello everybody, I am investigating the influence of a toxinon various clinical-chemical and haematological parameters in fish. I have three treatments (control, low dose, high dose) in my...

24 February 2021 1,243 1 View

Is it harmful to breed for Cre Homozygosity initially?

Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...

24 February 2021 5,759 3 View

How to empty large intestine in mice ?

Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...

23 February 2021 7,701 2 View