Urethane doses - sex and strain differences?

28 February 2019 0 8K Report

If you use urethane for anesthesia purposes in mice, can you share differences you have noticed between sexes and strains? We are noticing females are more sensitive (C57 to some degree, but majorly so in DBA2 mice). Similar experiences?

Badges
Science topic

Similar topics
Eutheria
Rodentia
Muridae
Murinae
Mice

More Deranda Lester's questions See All

What are the dimensions are using on his x Axis on this XRD spectra?

Hi. I'm looking the dimensions that are giving on this graphic on the "X" axis, as they are not giving it on 2(Theta) degrees. Can you help me to solve this and how to convert it to normal...

12 December 2023 3,020 2 View

Can I directly transform DpnI-treated DNA, or should I purify it first before transformation?

Hi All, can I directly transform DpnI-treated DNA, or should I purify it first before transformation?

06 October 2023 4,076 2 View

Formualtion of Bilastine with Beta-Cyclodextrin for pharmaceutical oral solution?

I'm currently working on the best way to solubilize Bilastine using Beta-Cyclodextrin. I have been reading a lot of Journals and Patents about it, but the best Journal I found...

12 March 2023 6,643 1 View

How does wave steepness H/L changes or influences the geometry of orbital motions in shallow water waves?

I must study this because I want to investigate about the cross-shore sediment transport due to waves in beaches. I am looking for, papers, books and documents that speak specifically about this...

21 June 2021 3,958 0 View

I sent on five new publications( 2 months ago) but have not seen them?

I sent on five new publications but have not seen under my name Dr. Lester Mandelker

10 June 2020 7,289 3 View

Do you have a certain management style in mind which would enhance this vision?

There has been much written about "teal" organization styled management -- are you considering teal as a possible enhancement that would favor transformational goals in society?

07 January 2020 1,990 0 View

How can I detect a drainage reversal?

Hello According to Harel et al (2019), a drainage reversal is represented by obtuse angle of a river junction. Does anyone know about an algorithm that can detect them? Best...

06 October 2019 6,175 2 View

To create a Chi-map, do I need to calculate an mn ratio per stream or can I use a general value per watershed?

Hello I'm currently trying to create a chi-map using topotoolbox for matlab. In the available literature most of the calculations use a single mn ratio (0.45-0.5) for the entire area, and some...

17 February 2019 7,065 4 View

What do you do when the set shear rate and the measure shear rate values are different during a measurement? Is there a correction that can be done?

I'm using a HAKKE MARS II rheometer. Im currently linearly increasing the strain by setting a fixed value for shear (strain) rate. My problem is for rates lower than 1/s, the measured values are...

13 November 2016 1,367 1 View

About center of gravity of an object (centroid)?

The center of gravity of an object(centroid) is the same if the object is represented by shape or contourn?

29 April 2016 421 10 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Why do men not accept that continually hassling for sex proves that they want it more than their partner?

Your partner’s not there to service you, it’s not their job to keep you sexually satisfied. You’re together because you love each other and want to make each other happy. Constantly hassling them...

08 August 2024 1,491 0 View

Mouse CD3 antibody sequence?

Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much

08 August 2024 9,157 4 View

How do I conformally do PR spin coating on trench structure?

I did PR spin coating on trench structure. I used AZ P4620 PR and the thickness or PR is around 11um. The substrate is Si. And my trench structure depth is 141um(negative way). Even though I...

08 August 2024 2,298 0 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

In what part of the brain´s rabbit bdnf is located and include references please?

I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....

04 August 2024 762 1 View

How can I find access to heavy metal reference doses from the EPA and WHO websites?

Reference dose and Maximum acceptable concentrations HMs

03 August 2024 8,230 4 View

Why do women not understand that men are aroused by physical contact?

Women often complain that their husbands never touch them unless they want sex. (Michele Weiner-Davis)

02 August 2024 7,778 2 View