Please excuse the poor photograph. This very large body has appeared in my mouse intestinal organoid culture. To the right (out of focus) is my budding organoid, and in the centre is this very large 'cell' with a thin membrane and some granular cell contents. Usually the mini organoids appear as small doughnut-like perfect spheres, which then grow bigger, with quite a thick row of cells around the edge, before they begin to bud and grow to the size as seen at about 1 oclock in this picture) Whatever it is, it is not showing any evidence of budding, and is not deterred by the pen/strep/gentamycin AND fungicide I have in the media. Anyone seen this before?
Hello Lira and thank you for such a quick and useful response!
I didn't see my imposter as a real threat, and having passaged the cells today I'm confident it has been broken up. Your reference has given me plenty to think about! Many thanks!
William, thank you for that insight- it leaves me with added worries as I have organoids growing well from a second mouse which are much less "polluted" with spheroids and I had thought the problem was solved. I am not studying these cells for cancer research, but the increased Wnt signalling is something I will have to try to overcome. Do you know if this is evident in human intestinal organoids, or limited to mouse?
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