Under which filter should I view acridine orange and Etbr staining for checking cell viability of cancer cells?

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Shweta Umar

can i perform Etbr/AO staining to my treated cancer cell cultured on 12 well plate ? anyone please provide a full protocol for staining and detection

Mehran Hariri

You can do ethidium bromide and acridine orange staining in 12 well-plates. The principal is the same. Just change the seeding cell number and stain volume.

Take a look at Ribbe et al. 2005 paper for complete protocol

Article Chapter 20. A Simple Technique for Quantifying Apoptosis in ...