Unable to get transformants in MATalpha strain of Saccharomyces cerevisiae, please suggest me why?

Partha Dey @Partha-Dey-12

18 November 2022 1 4K Report

Dear yeast biologist,

I performed 4-5 time yeast transformation in S. cerevisiae SK1 MATalpha strain but I am unable to get transformants.

I performed and checked all the necessary steps as per my knowledge.

If possible please suggest me, how to troubleshoot the problem?

Satyendra Mondal

Dear Partha Dey

It would be easier to understand your trouble if you mention which type of protocol you are following and which type of DNA (plasmid or DNA cassette, its size and amount), selection marker (antibiotic or auxotrophic) etc. with your question.

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