Dear all,
I have a naive question. When we want to study Type 1 response, we infect mice with influenza for instance, if we want to study Type 2 response we use for instance T. Bruecei. Does anybody know which type of response we induce when we immunize with our classical protocols such as SRBC or NPCGG/NPOVA/NPKLH?
Thank you very much
From an old hand at immunology, a Type 1 response is what we used to call a T-dependent antibody response, and that is triggered by viruses, SRBC and hapten-carrier conjugate. Basically, the B lymphocyte binds the relevant antigen [in your case, NP], endocytoses it and processes the carrier protein for display on its' surface class II MHC molecules. If a T helper cell recognizes the carrier protein antigen that is displayed, it binds to the B lymphocyte and stimulates it to produce antibody. For naive B lymphocytes, you get IgM initially, but IgG later in the response. Classic immunology.
Type 2 responses are T-independent; the carrier is LPS, or Ficoll or T bruecei. These are or have repetitive structures that bind to the B lymphocyte and crosslink the surface immunoglobulin [IIRC, you need a complex of at least 10 hapens to trigger the response and Toll-like receptors are involved]. For T-independent responses, you get primarily IgM, and some IgG3 in mice.