Is there a preference when prepping libraries for column purification over solid phase paramagnetic beads?
I'm looking for a protocol for dissociating tissue into single cell suspension. Downstream application is high molecular weight DNA extraction. I'd prefer enzymatic dissociation (collagenase) then...
11 December 2017 3,231 0 View
I'm using Agilent HWM DNA extraction kit. PFGE shows a smear starting at around 50kb and going up. However, I want >50kb and preferably 100kb and up. Pippin size select only goes to 40kb. Does...
31 December 2016 4,520 6 View
see above
02 March 2014 1,064 0 View
What is a good gene to normalize to in RT-qPCR if I'm exposing tissue/cells to toxin that globally alters transcription?
08 September 2013 5,055 8 View
RT-qPCR is a gold standard for measuring mRNA abundance of a targeted gene, and it has largely replaced nuclear run-on assays. While there may be some overlap among the two assays, a nuclear...
08 September 2013 3,205 1 View
Are there biased regions? Does anyone have experience with a particular brand such as Sigma's WGA.
31 December 2011 7,489 3 View
What is the rationale for using T4 DNA Polymerase with and without a DNA polymerase Klenow Large Fragment for creating phosphorylated blunt ends? It seems only cloning protocols call for the...
31 December 2011 2,949 12 View
Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...
06 August 2024 1,495 2 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...
05 August 2024 6,247 3 View
kindly reply me. Thanking you in advance.
05 August 2024 7,727 4 View
Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...
03 August 2024 3,371 3 View
I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?
02 August 2024 3,143 4 View
Molecular docking software/ websites?
02 August 2024 8,704 7 View
Dear All Gromacs User I would like to restart MD without using checkpint .cpt files? I heard there is a way for acheiveing this. Any input and sugegstions are most welcome. Thanking You Budheswar
29 July 2024 3,084 8 View
I created a file with my outgroup and ingroup species using Beauti, ran it in BEAST, viewed it in Tracer, and then used TreeAnnotator to create a file that I imported into RASP. Could someone...
28 July 2024 2,979 1 View
PeptiCloud (www.pepticloud.com) is a bioinformatic platform that allows researchers to organize and share their data for their projects as well as collaborate with others in one place. Through...
28 July 2024 4,762 2 View