TruSeq DNA Sample Prep Protocol

01 January 2012 0 6K Report

Is there a preference when prepping libraries for column purification over solid phase paramagnetic beads?

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Protocol for single cell suspension from fish tissue?

I'm looking for a protocol for dissociating tissue into single cell suspension. Downstream application is high molecular weight DNA extraction. I'd prefer enzymatic dissociation (collagenase) then...

11 December 2017 3,103 0 View

Extracting HMW DNA for 10x genomics lib prep: How do I size select for 100kb and up fragments?

I'm using Agilent HWM DNA extraction kit. PFGE shows a smear starting at around 50kb and going up. However, I want >50kb and preferably 100kb and up. Pippin size select only goes to 40kb. Does...

31 December 2016 4,361 6 View

Is there any evidence of early embryonic (0-52hr) cardioprotectiveness in Zebrafish that diminishes around 72 hours?

see above

02 March 2014 888 0 View

RT qPCR interntal control for global transcription deregulation?

What is a good gene to normalize to in RT-qPCR if I'm exposing tissue/cells to toxin that globally alters transcription?

08 September 2013 4,927 8 View

mRNA abundance versus gene expression?

RT-qPCR is a gold standard for measuring mRNA abundance of a targeted gene, and it has largely replaced nuclear run-on assays. While there may be some overlap among the two assays, a nuclear...

08 September 2013 3,084 1 View

Genome wide amplification polymerase

Are there biased regions? Does anyone have experience with a particular brand such as Sigma's WGA.

31 December 2011 7,301 3 View

End repair (blunt ends)

What is the rationale for using T4 DNA Polymerase with and without a DNA polymerase Klenow Large Fragment for creating phosphorylated blunt ends? It seems only cloning protocols call for the...

31 December 2011 2,711 12 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View