James Anthony Gill

8 Questions 37 Answers 0 Followers

Questions related from James Anthony Gill

Protocol for single cell suspension from fish tissue?

I'm looking for a protocol for dissociating tissue into single cell suspension. Downstream application is high molecular weight DNA extraction. I'd prefer enzymatic dissociation (collagenase) then...

12 December 2017 3,194 0 View

Extracting HMW DNA for 10x genomics lib prep: How do I size select for 100kb and up fragments?

I'm using Agilent HWM DNA extraction kit. PFGE shows a smear starting at around 50kb and going up. However, I want >50kb and preferably 100kb and up. Pippin size select only goes to 40kb. Does...

01 January 2017 4,482 6 View

Is there any evidence of early embryonic (0-52hr) cardioprotectiveness in Zebrafish that diminishes around 72 hours?

see above

03 March 2014 1,037 0 View

mRNA abundance versus gene expression?

RT-qPCR is a gold standard for measuring mRNA abundance of a targeted gene, and it has largely replaced nuclear run-on assays. While there may be some overlap among the two assays, a nuclear...

09 September 2013 3,166 1 View

RT qPCR interntal control for global transcription deregulation?

What is a good gene to normalize to in RT-qPCR if I'm exposing tissue/cells to toxin that globally alters transcription?

09 September 2013 5,017 8 View

TruSeq DNA Sample Prep Protocol

Is there a preference when prepping libraries for column purification over solid phase paramagnetic beads?

01 January 2012 6,481 0 View

End repair (blunt ends)

What is the rationale for using T4 DNA Polymerase with and without a DNA polymerase Klenow Large Fragment for creating phosphorylated blunt ends? It seems only cloning protocols call for the...

01 January 2012 2,879 12 View

Genome wide amplification polymerase

Are there biased regions? Does anyone have experience with a particular brand such as Sigma's WGA.

01 January 2012 7,418 3 View