Brief protocol:

- Mice were fasted for 2 hours

- Mice were gavaged with 200uL of intralipid +4uL of 3H-triolein (2uCi), wait 2 hours

- euthanize, harvest adipose tissue

- tissue subjected to chloroform methanol lipid extraction, (Folch method)

- Aqeuous phase and organic phase seperated into 2 scintillation vials

- counted on a liquid scintillation counter.

Question:

According to the Folch method the organic (bottom) phase contains the lipids, while the aqeous phase contains everything else, so the 3H-triolein (triglyceride) should be present in the organic phase and thus have higher 3H count, however the counts show the opposite, where the aqueous phase has higher 3H counts than the organic, is there a reason for this or did something likely go wrong?