Hello everybody,

I am using transwell migration assay with inserts of 6.5mm diameter to determine cell migration. After some incubation time, cells get stained to be able to count under microscope.

Calculating the percent of migrated cells, certain factors are needed: the Filed number and the magnification to determine the diameter of the measuring field (wherein the cells are counted).

I also know, that I have to divide the counted cells by the field area and than multiply by the area of the used insert.

The company is giving an example: (210/0,001cm²) x 0,33cm².

210 is the amount of cells, 0,001cm² the area of measuring field, 0,33cm² the area of the transwell insert.

I am struggling now with the 0,001cm², since they did not provide any information to the field number and magnification they used.

Calculating backwards with a commonmly used Field number of 2.2 cm and the common formula for circle area A=π·r² , I come to a magnification of 61.1X (??)

While reading through various papers, most only used the diameter of the measuring field instead of the area of measuring field. Thats why I am also unsure, how it might be mathematically correct

I am veryy happy if you explain your opinion or your approach to me :)

Best regards :)