What is the smallest Archaeological feature size that has been successfully analyzed using either LIDAR or Satellite Image analysis?

I am considering the viability of using AI in conjunction with either LIDAR based Digital Elevation Models or Object based image analysis of Satellite Imagery to predictively locate Inuit...

25 October 2020 9,525 2 View

What is a typical DBC of an affinity column?

I'm purifying TB antibody and the columns we are using only seem to have a DBC of about 0.15 mg/ml. This seems very low to me, but I am a protein purification novice. Is this normal?

14 July 2020 1,443 4 View

How do anthropogenic pressures influence and will influence coastal groundwater according to the climate and its evolution?

Groundwater is of utmost importance in Haiti. It is the main source of drinking water for the Haitian population. The degradation of the quality of this resource is a major issue in the context...

29 June 2020 8,626 6 View

No title

Whether for a dissertation, a thesis or any other research work, there will be a problem in which it is clearly posed what is causing the problem and which imposes on the researcher the need to...

29 June 2020 4,902 9 View

What is the best model for this dataset?

Hi, I'm trying to do some IoT device classification, the dataset I'm using looks like this: 100 classes 5000~15000 samples for each class 10~15 features per sample Can you suggest the most...

16 April 2020 1,719 9 View

What would be standard formula/equation to derive post-test mean & standard deviation values from the established study for meta-analysis?

Since in some studies, authors only report the mean difference and mean difference standard deviation. Kindly, help me out to get the absolute mean and SD of post-test measures. Thanks

22 March 2020 5,358 7 View

How to purify large amounts of plasmid after restriction digest?

03 November 2019 2,686 4 View

Why cell proliferation increased after change amino acid by SDM?

Dear Scientists, I am working with a gene of interest in colorectal cancer, which has no role in cell proliferation. In addition, by knockdown this gene, the FAK and SRC protein expression are...

09 October 2019 6,257 3 View

I did emulsion polymerization using 2-carboxyethyl acrylate as co monomer. When I increased the amount of CEA, particle size increases. Any idea?

I did emulsion polymerization using 2-carboxyethyl acrylate as co monomer. When I increased the amount of CEA, particle size increases. I found out that at higher CEA content, particle size is...

03 July 2019 4,764 4 View

Does anybody know a company specialized in platinum machining?

Good day! Does anybody know a company providing on-demand platinum machining service? Thank you in advance for your help! Kind regards, Reda

14 February 2019 9,275 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View