Training and the environment?

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AAS dried plant samples digestion protocols?

I am looking for alternative sample digestion protocols for dried herbs to be analyzed with AAS for presence of Pb, As and Cd. So far I have attempted acid digestion over hot plate (HNO3 and aqua...

28 December 2020 7,765 5 View

How to draw ROC curve for the correlated parameters?

Dear Fellow Researchers, We want to access the prediction accuracy for various parameters. We were able to draw ROC curves for each of the parameter separately using SPSS. Now, we want to see a...

21 October 2020 4,994 3 View

How can I analyze qPCR data without control sample/calibrator?

I would like to compare expression of my gene of interest (GOI) in non-treated leaves, stems, roots of my plant. In that case I don't have any control sample here and it's not possible to have...

09 August 2020 3,998 3 View

Is it possible that dicot plant could rearrange or excise from his genome transfected DNA fragment?

I have transfected Salvia milthiorrhiza plant using Agrobacterium tumefaciens cells carrying binary plasmid. At the level of Agrobacterium I confirmed presence of my binary vector by obtaining...

26 June 2020 8,763 15 View

Where can I find suppliers of rare dyes?

I have trouble finding certain dyes for my research (mostly acid dyes). I have approached producers of textile dyes from various countries, but the C.I. I am looking for are extremely hard to...

26 March 2020 5,648 6 View

MatLab -curve fitting?

Dear All, Could anyone help me please? I want to estimate the contribution of each curve (EA) to best fit the M curve (the bold one). Best regards Malgorzata

24 March 2020 8,316 5 View

Any anti-HLA-B38/B39-specific antibodies?

Dear Fellow Researcher, I am working on HLA-B*38 and HLA-B*39 molecules and looking for the antibodies that can recognize these two MHC class I alleles. I do have W6/32 and HC10 antibodies that...

02 December 2019 6,693 1 View

How to evaluate the quality of the studies included in the systematic review?

Dear Fellow Researchers, We are writing a systematic review based on the various types of the studies: clinical trials, clinical case series and the animal studies. What type of study assessment...

17 November 2019 3,131 3 View

Which kit would you recommend for isolation of RNA from dicotyledonous plants (leaves, stems and roots)? Which vendor would you recommend generally?

My plant material is rich in secondary metabolites. With the isolated RNA I would like to examine expression of interested genes in Real Time PCR.

12 November 2019 5,338 8 View

How to efficiently transfer 1.5mm SDS gel on PVDF?

Hello, I have issues with transferring 1.5mm thick SDS-PAGE gels (14% acrylamide, with saccharose) on PVDF membrane. I tried 50 min @ 100V in Towbin buffer with 10% Met-OH, but most of the...

02 July 2019 1,898 4 View

Dear all, I was wondering if anyone knows whether MGLtools and Autodock can run on Mac OS?

I have tried to download the 64-bit version of MGLtools however, as many times as I have downloaded and uninstall the programs, an error arises that the app needs to be updated. Thank you.

03 March 2021 5,997 2 View

I got 02 moderator values defining Johnson-Neyman significance regions. Is it an error?

Hi everyone, I am conducting research for my Master's thesis. I am using PROCESS by Johnson-Neyman to analyze my Moderator model. I test the relationship between Public Service Motivation and...

03 March 2021 2,350 2 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Why are the velocities so high in my Boussinesq approximation Fluent simulation?

Hello, I have a mechanism where air rises due to convective flows. In this simulation, nothing is driving the air except this. I understand that the Boussinesq approximation only works for small...

02 March 2021 4,910 3 View

Do I need to strip the PVDF membrane before staining it with colloidal gold?

Dear Colleagues, After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on...

02 March 2021 7,829 3 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Can NFL theorem be valid in infinite search space in ML?

NFL theorem is valid for algorithms training in fixed training set. However, the general characteristic of algorithms in expanded or open dataset has not been proved yet. Could you show your...

01 March 2021 1,189 3 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Is there any research regarding how training and development is going to change in the era of Fourth Industrial Revolution?

As the organizations getting prepared for the fourth industrial revolution, to enhance the skills of their workforce what type of changes are necessary and going to occur in the training and...

01 March 2021 3,199 3 View

OpenBCI eeg--has anyone used them?

I'm looking to try out an off-the-shelf EEG set for some exploratory work. Curious if anyone has used the OpenBCI, and how much set-up work is required. I've heard they are more difficult to get...

01 March 2021 4,128 3 View