Total RNA extraction?

06 June 2014 0 9K Report

I am extracting total RNA from leaf samples using Purelink Plant RNA Reagent, followed by DNAse treatment and RNA clean-up according to Qiagen RNeasy kit protocol. On analysing my samples on bioanalyzer, the RIN is more than 7 and I get sharp peaks for 18s and 28s, but peak for 18s is higher than for 28s, 28s/18s ratio is less than 1.0.

Has anyone else faced the same issue and any suggestions about how to get over this? Or would this sample be good to make cDNA?

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