We purified RNA from plant leaves and got only one band on agarose gel electrophoresis. Anyone khonw`s the reason of it?
Dear mehmet;
Thank you for your response. I used Qiagen Rneasy plant mini kit. For agarose gel electrophoresis we used TBE buffer. This is my last gel picture.
Best regards
Dear Mehmet
I used only TBE buffer and 1% agarose. TBE buffer was diluted in sterile deionized water. We used FluoroDye for staining.
Regards
I will try and inform you. Thank you so much.
sincerely
What will happen if we load too much of material on gel? We used almost 5 microliter of extracted RNA with the concentration of 200 up to 500 nanogram.
Sincerely yours
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