Contemporary LGBTQ+ literature/writers in Ecuador?

I am looking to find contemporary (2005 and onward) LGBTQ+ literature/texts (novels, poems, digital literature, correspondence, social media writers) by Ecuadorian writers (preferably LGBTQ+ too,...

04 February 2020 9,780 3 View

What are the risk and resilience factors for student mental health?

Students in their final year of a Psychology degree at the Royal Holloway University London are researching factors affecting student mental health. All students studying in the UK please...

27 November 2019 241 2 View

Best experimental setup for ROS measurement after plant extract treatment using RAW 264.7 and H2DCFDA?

I am treating RAW 264.7 macrophages with some plant extracts dissolved in ethanol and measure if they decrease ROS/NO production. I have been having some trouble measuring ROS. A condensed...

20 August 2019 9,643 3 View

Why do no bands appear during glycoprotein staining?

Dear all, I'm trying to stain sugar groups attached to my protein with Glycoprotein staining (Thermo Scientific Pierce Glycoprotein Staining Kit #24562) after gel electrophoresis. I've added 4...

07 April 2019 3,224 2 View

How do I know that I can trust the chemical analysis results of surface and ground water quality samples from a subcontractor?

I was unable to perform the sampling and analysis for 40 surface water and 20 groundwater samples, so I hired an in-country consultant firm to do it. The results that they returned were difficult...

21 October 2018 8,937 10 View

Can anybody help me? Why is the genetic variance negative?

12 September 2018 1,959 14 View

Why can't we mate Thy-1 GFP to other transgenic lines?

We have a Thy-1 GFP mouse in our colony. We maintain the mouse as homozygous because the heterozygous animal did not show a strong enough signal. There doesn't appear to be any issues with the...

29 August 2018 3,576 4 View

What is the typical duration of a cell invasion assay? 24-48 hours, longer?

Can cell migration/invasion in the presence and absence of pharmacological inhibitors be tested for 1 week using this assay?

01 April 2018 5,885 5 View

Problems with protein purification by ion exchange chromatography

Hello everyone, I am trying to purify my protein (enzyme) expressed in Pichia pastoris through ion exchange chromatography. The protein is extracellular, so I extract the supernatant after...

27 July 2017 4,012 5 View

Ellipse on PCA analysis

Can I ask what the ellipse argument is used for in PCA? How to analysis the data by different ellipse shape and different direction in statistics? Is there any example for interpretation data by...

30 May 2017 7,219 1 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

Image Processing and classification using keras/Tensorflow?

Do you have some recommendations approaches on Image processing and classification of plant diseases using TensorFlow/Keras?

02 March 2021 1,493 4 View

Which SAR dataset to use for generating coherency matrix and performing target decomposition (Description) ?

Working on chandrayaan-2 DFSAR data, there are three datasets available: 1) Slant range image data product: The slant range complex image file. Each pixel is represented by two 4-byte floating...

02 March 2021 8,481 3 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View

How could I process data in RCT to include it in meta-analysis?

I'm in the process of doing a meta-analysis and have encountered some problems with the RCT data. One of my outcom is muscle strength. In one study, I have three different measurements of muscle...

25 February 2021 7,603 3 View

What is the suitable range of Sulfur content and Viscosity of Heavy fluid Oil that used in Kiln?

What is the disadvantages of high sulfur content to the ceramic burning process? and what is the effect of outranged viscosity?

24 February 2021 8,219 2 View

Can anyone please help me in processing SAR SLC data to generate interferogram using SNAP?

I have two scenes of the study area, one top and the other bottom. As per the tutorials and papers published, the step include coregistering the master sand slave images. However, when i try to...

24 February 2021 6,372 1 View

What is the optimum time and temperature of blanching and pre-frying potato for frozen fries?

I am helping a local home industry for developing frozen fries from their local farm. I want to know what is the optimum blanching and pre-frying temperature and time for processing. Please also...

23 February 2021 1,616 5 View