Tomato fruit quality analysis protocol?

More Giulia Antonucci's questions See All

Staining of foam cells derived from PBMC differentiated to macrophages. Does anyone have experience with this type of assay?

Hi everyone, I am trying to induce foam cells in macrophage differentiated from PBMC. Which type of staining do you prefer to use? Nile Red or Oil RED O ? What concentration and timing of ox-LDL...

01 July 2024 8,286 0 View

Which negative control do you recommend for In vitro Foam Cell Formation Assay?

Hi everyone I have to perform the Foam Cell Assay with OIL RED O staining. Can you recommend a compound that inhibits LDL oxidation? Also, can anyone recommend oxLDL to use?

14 May 2024 6,404 1 View

Can I transfect differentiated cells?

In particular using a DNA plasmid transient transfection on differentiated skeletal muscle cells?

29 April 2024 9,064 2 View

Research article about minors community?

i need some information to build a thesis about minors community

22 April 2024 1,081 0 View

What do you think I'm doing wrong?

Hi everyone, I differentiate THP-1 in macrophages with PMA 48h + 24h rest. After treatment with LPS I would expect an increase in ROS. But this increase compared to control I cannot get. I have...

25 March 2024 4,797 6 View

Which statistical test should I use?

Hi, I need some information. After collecting data from various experiments, I am proceeding with a statistical analysis. Since I work with frequencies, I was told to use the chi-square test....

20 March 2024 5,496 4 View

When does the Griess colorimetric assay produce yellow instead of pink?

I am using the Nitrate/Nitrite Colorimetric Assay Kit from Cayman. When I used the nitrate+nitrite determination assay with nitrate reductase on the same samples' supernatant, it turned pink. When...

18 February 2024 3,338 0 View

Hello there, any suggestion to calculate equilibrium constants without NMR variable T?

If we have an host-guest complex, in water an equilibrium occurs. So you can have ComplexGuest + H2O ComplexFree + GuestFree. So I can calculate equilibrium constant, but in the most of...

31 January 2024 335 1 View

What medium can I use (instead of RPMI) on differentiated THP1 if my goal is to measure small changes in nitrate levels?

I differentiated THP1 cells into macrophages. Subsequently, macrophages will be treated with substances. Next, the Griess Reagent test will be used to determine nitrate levels. The Griess kit...

24 January 2024 9,551 1 View

Why are my articles linked to another account?

I've registered on ResearchGate today but I can't upload my articles because they are linked to another account with the same name: Giulia Errico, Zurich University of Applied Sciences,...

29 October 2023 1,270 1 View

Leaf area of tomato ?

Hi How can this equation Ln(LA) = 1.038 + 0.89 ln(X) be applied to calculate the leaf area of a tomato? Can you explain with an example and what is the substitution of Ln and ln?

06 August 2024 2,508 2 View

What are the best practices for washing and preparing fruits and vegetables to reduce pesticide residues?

27 July 2024 9,147 4 View

Why microbes are used in environmental engineering and role of microbes in reducing environmental pollution and improving environmental quality?

25 July 2024 5,192 5 View

What are the reasons behind my contradicting sample concentration using HPLC analysis?

I have conducted a study on the fructose, glucose, and sucrose compound content in tomato flesh extracts. The obtained peak areas aligned with the chromatogram graph. However, the concentrations...

19 July 2024 449 2 View

How does the digestate compare to other organic amendments regarding quality and performance in different countries?

Experts are welcome to share their experience country-wise so that we can make a nice document listing all the country-wise experiences. ? How does the digestate compare to other organic...

18 July 2024 5,225 3 View

What does an additional peak in FTIR between 3100 and 3300 nm affect the quality of the material, benzethonium chloride USP??

I do not know much about FTIR! The material Benzethonium Chloride USP was compared to a reference standard in regards to FTIR, as per the USP testing: Identification B. Infrared absorption (FTIR),...

16 July 2024 6,918 7 View

Is it appropriate to apply the COSMIN guidelines for evaluating the quality of tools used to assess acute postoperative pain?

Hi, I'm a graduate student, college of nursing, chungnam national university in Daejeon. The topic of my thesis is "Quality Assessment of Tools for Evaluating Acute Postoperative Pain." I have...

10 July 2024 3,048 1 View

Increasing Area Ratio on Quality control Standard on LCMS ?

Hello everyone. During my analyses on LC MSMS, the area ratio (Standard Area/Isotopically labelled Standard Area) increases with time for the quality control point. I'm having trouble explaining...

08 July 2024 3,150 2 View

Can type 2 diabetes be reversed at any age through proper diet and exercise?

Type 2 diabetes can be managed and, in some cases, put into remission through proper diet and exercise, regardless of age. Here are some key points: Weight Management: Achieving and maintaining a...

07 July 2024 8,745 2 View

Does produce in season have more nutrients when eaten in season and fruits and vegetables safe to eat if they have been treated with pesticides?

02 July 2024 4,144 2 View

Rajesh Dev Sarkar

Dear @Giulia Antonucci, I am not aware of Brix and TSS... Bit Lycopene estimation from Tomato is an easy and rapid process, you can easily get the protocol... Still I am adding a paper for you.. take the precautions necessary to handle Lycopene.

Giuseppe Perretti

This is one of our experiences on tomato products and quality measurement.