Toluidine blue protocol ?

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Respected academician, I did staining of dormant and non-dormant seeds. Dont know how to explain these pics? please help ?

Peroxidase activity 📷📷 Dormant seed Non dormant seed

17 April 2024 7,384 2 View

Cultural validation of a tool for immigrants?

Hi, I have an inventory designed for USA . I want to use it for immigrants in UK. Should I have to validate it for each of subgroups like for Indians, Chinese , Thai and etc of my selected population?

28 January 2024 6,744 2 View

How do you calculate the fractional inhibitory concentration index of two combinations of antibiotic and plant extract, when their MICs are unknown?

For example, antibiotic X's MIC is reported as >32 µg/ml and MIC plant extraction is reported as >10 µg/ml. what value should we used to calculate the FIC according to the equation?...

07 August 2023 9,626 3 View

What would be the best post hoc analysis following the significant three-way interactions in ANOVA using linear mixed effect model?

In my study, I have ten dependent variables (DVs) and three independent variables (A.B, and C) *A (2 levels) *B (3 levels) *C (2 levels) My research question is how the three-way interactions of...

27 July 2023 3,455 4 View

What is the difference between hypothesis and predictions in a research study? Are there any good resources to follow for writing good hypothesis?

I am currently conducting a research study on the microbial N-cycle, and I would like to create some reasonable hypotheses for my research. However, I sometimes find it challenging to distinguish...

15 June 2023 3,667 1 View

How can chromosomes be isolated for observation using a microscope?

I treated cultured cells with Colchicine (0.5ug/mL) overnight, isolated the cells, and treated them with hypotonic solution (0.075M KCl). After stopping the reaction with Carnoy's fixative...

06 April 2023 10,069 0 View

What is the optimal sample size for criterion validity (To compare a 36 item PA scale with a pedometer)?

I am adapting and validation YPAS to Sri Lanka. Need to know the optimal sample size for criterion validity of YPAS by comparing it to Omron HJ0325 pedometer. TIA

03 April 2023 3,712 3 View

Using years in mutiple regression?

I have dengue incidences as outcome and as independat nvariables time, rainfall, wind flow. Time is as years. Can I do multiple regression for that

17 December 2022 5,606 4 View

Can I use sen slope to analyse trends in median prices?

I have a data base on price changes over years.Can i use sen slope to analyse trends in median prices?

22 September 2022 630 2 View

Are there any validated tools to assess 7 features of effective communication?

I am looking for a tool to assess effective communication 7 pillars in a communication of physicians. Is there any validated tools?

30 November 2021 1,236 3 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Do I need specific antibodies to stain for a fusion protein?

I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...

07 August 2024 5,338 2 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

How to quantify polystyrene microplastic (8 micron) bioaccumulation in fish tissue?

"I have treated adult zebrafish with 8-micron polystyrene microplastic and want to study the bioaccumulation in different organs. Can this be done using hydrogen peroxide digestion followed by...

05 August 2024 853 3 View

ANY free software for reconstructing neurons in the microscopic image?

Hi everyone, I am working on brain slices for visualizing a protein in the soma and dendrites, using a fluorescence tag. However, I need a tool (not paid) for reconstruction of the whole neuron,...

04 August 2024 4,725 2 View