Hey Ahmad,

What do you mean by feeding strategy?

Hi Daniel

I mean controlling pH by glucose concentration, which itself can be managed by flow rate of feed

You mean a fed-batch, feeding cells low amount of glucose at a time, to minimize lactate production?

I believe with that strategy you could lower the lactate accumulation in culture medium. However, I would say that even so after a while you would need to lower the pH by other method (base addition, CO2...)

But I am sure there are plenty of articles with glucose fed-batch. Try to find some with your cells or similar ones. Good luck!