In some of the lines of maize we found the nitrogen fixing bacteria. to validate the finding what can I do? which parameters i should take to analyse in the field as well as in laboratory with DNA or marker analysis?
Thank you so much sir. Sir if I am not wrong then the nif gene is present in bacteria so we can find it out by isolating the bacteria from plant material and then for evaluation of nitrogen fixation and assimilation we have to compare the nitrate reductase, GS enzyme activity, GDH activity in the fixing and non fixing plants. Will this be the right way to proceed? @Anoop sir.