To propagate high titers of Rhinovirus, is it necessary to reinfect H1Hela cells multiple passages prior to harvest or scale up & concentrate?

13 March 2018 0 3K Report

I have just started culturing Rhinoviruses and some protocols suggest they infect and then use that to reinfect H1Hela cells upto 5 times prior to viral isolation and concentration. Since it is a lytic virus not sure what this accomplishes or am I missing something? I need high titers. Do I just scale up the number of culture dishes and then concentrate it down?

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