To propagate high titers of Rhinovirus, is it necessary to reinfect H1Hela cells multiple passages prior to harvest or scale up & concentrate?

I have just started culturing Rhinoviruses and some protocols suggest they infect and then use that to reinfect H1Hela cells upto 5 times prior to viral isolation and concentration. Since it is a...

13 March 2018 2,691 0 View