I found a wide range in different papers or company websites ranging from 40 uM to 100 uM. Does using 100 uM will have cells in clumps and using 40 uM we may loose bigger cells? However, I found on Research Gate discussion that the size of cells of spleen vary in the range from 5-10 uM only. So getiing confused why we need 100 uM strainer.

Thanks.