Sarika Chaudhari

4 Questions 6 Answers 0 Followers

Questions related from Sarika Chaudhari

Is snap freezing of supernatent cell culture media recommended?

Which samples should be snap freezed? Should we snap freeze the cell culture media / supernatent from the cell culture dish ? or we can directly store it at -80 degrees. I am planning to do Human...

06 September 2017 1,839 1 View

Single cell suspension of Kidney. Getting low counts.

Hi all, I am working with single cell suspension of kidneys by enzymatic digestion for the first time. I did following steps. 1) chopped the kidneys, passed through 100uM cell strainer and...

16 November 2016 9,905 1 View

To make a single cell suspension of splenocytes what size of cell strainer to be used ?

I found a wide range in different papers or company websites ranging from 40 uM to 100 uM. Does using 100 uM will have cells in clumps and using 40 uM we may loose bigger cells? However, I found...

12 October 2016 4,113 2 View

Can I Freeze Bone marrow cells in DMSO and use for macrophage culture and subculture later on?

Hi all, I will be working on macrophages for the first time. I have few queries. Can bones, after dissection,be kept at 4 0C for 1-2 days before isolating the cells? or I should isolate the BM...

10 October 2016 9,946 12 View