To improve safety in what aspect of the company?

How is the bacterial genome's high protein count verified as genuine despite 800+ contigs and good metrics (98.55%completeness, 0.68% contamination)?

Given that the bacterial genome has over 800 contigs, but its quality metrics are good, with a completeness of 98.55% and a contamination of 0.68% as assessed by CheckM, what specific validation...

01 August 2024 1,514 1 View

Cloning 1334 bp insert into 6.1kb vector using HiFi DNA assembly/Gibson assebmly cloning ?

Hello, I am trying to clone 1334 bp insert into 6.1 kb vector using HiFi DNA assmebly/Gibson assembly kit. I am taking 100ng of vector and 1:5 molar ratio of vector: insert and 4 hrs of...

27 June 2024 9,652 2 View

To do gibson assembly cloning can I use DH5alpha strain of E. coli. for transformation?

20 June 2024 774 1 View

Why I am not getting colony after transformation in Dh5alpha?

I am trying to do gibson assembly cloning. I have two fragments for gibson assembly cloning (plasmid backbone and insert). After gibson assembly reaction I have transformed this in E. coli....

19 June 2024 6,404 4 View

Can anyone suggest an adhesive that can sustain 600C?

I have to join tungsten wire to metallic plate with that adhesive and entire assembly has to be heated upto 600C in ultra high vacuum. I know about zirconia paste but it is not readily available...

18 June 2024 3,092 3 View

Is it possible to use Overhangs with large different Tm with NEB Builder HiFi DNA assembly?

Dear cloning community, Is it possible to use Overhangs with large Tm difference with NEB Builder HiFi DNA assembly? 5´ 26 bp Tm 57ºC 3´ 24 bp Tm 72ºC Unfortunately, extending the 5´ doesn't...

17 June 2024 8,552 2 View

How to create Component Model Synthesis in Ansys Workbench?

I have an assembly of parts. I want to reduce the number of modes of the individual parts by CMS.

11 June 2024 2,966 0 View

How can I improve Gibson Assembly's efficiency?

In my undergraduate research project, I require an integrative plasmid containing GFP with an antibiotic resistance that can't be kanamycin. In our lab, all of our integrative-GFP-containing...

14 May 2024 9,861 3 View

Does anyone know how to do genome assembly for bacteria? Please share it if there is any online tutorial?

I would like to do genome assembly for the bacteria isolate from the environment. Can any provide me the information or any tutorial? It would be helpful! thanks!

18 April 2024 1,894 12 View

Can i keep distinct assembly parts after running topology optimization in Abaqus?

Hi! Does anyone know if you can keep the different parts of an assembly separate after running topology optimization on it in Abaqus? All three parts of my assembly merge to one after I...

17 April 2024 6,808 3 View

Francisco Rebelo

Hi Jill,

The project is related with distribution. At this moment, due to confidentiality issues we cannot spread the results.

Best

Jill Thorne

thank you. Please include me when you can share your findings.

Sure, thank you for you interest.

Ivan French

I would be interesed in any aspects related to moving and handlng of loads.

Bruno Durand

Hello,

You can improve safety in many aspects of a company : supply operations, industrial operations, co-packing and co-manufacturing, inventory operations and warehousing, handling, trucks loading, shipping and distribution...The first performance lever of a modern company (4.0 firm) is, indeed, the staff safety!