Hi Dewi, if you’re looking for a positive control for antioxidants, I would suggest to use vitamin c (ascorbic acid), as it is more commonly used in general cell culture in comparison to vitamin e (usually used for stem cell differentiation protocol). But I would also like to suggest for you to have another group of increased serum concentration (if you’re looking to compare proliferation rate) as I do not believe that vitamin c can drastically increase the growth rate. Good luck!

Hello Ahmad Khairul Aizad Ahmad Khairi thank you very much for your answer, the answer is very helpful

I hope you always stay healthy~

Hello Dewi Murda,

The choice of which vitamin to use as a positive control depends on the specific research question and the type of sample or system being studied.

Both vitamins are important antioxidants and can serve as effective positive controls in studies investigating antioxidant activity. 

But there is a differrence!

Vitamin C might be a better choice for studies involving water-soluble antioxidants or aqueous environments,

while vitamin E might be a better choice for studies involving lipid peroxidation or lipid-rich environments. 

Since alfalfa plant extract contain water-soluble substances, you should select vitamin C as the positive control for your study.

Best,

hai Malcolm Nobre thank you very much for your answer, this answer so helpful for me~

i hope you always stay health