Dewi Murda

7 Questions 14 Answers 0 Followers

Questions related from Dewi Murda

Result of crystal formazan from MTT assays?

I did an MTT assay test, then gave the MTT reagent to my cells, then during the observation after giving the MTT reagent, purple granules were seen as in the picture. Is it true that formazan...

23 June 2025 905 3 View

How to calculate LC50 for toxicity test?

I performed an MTT assay to determine the viability and toxicity values of cells. For analysis of MTT assay results, readings were performed using an ELISA reader. How do I determine the LC50...

21 May 2025 5,722 2 View

Analysis of the results of observations of fibroblast cells under a microscope?

This is the result of observation of fibroblast cell culture that was given 5% extract treatment after 3 days of incubation. Can you help me analyze the image? I am doubtful whether my culture has...

29 April 2025 6,719 4 View

Why did my culture grow mold (makroskop) and looks like crystal (mikroskopis)?

I did the implantation of fibroblast cells isolated from the back skin of baby mice. The growth medium was given additional treatment in the form of ascorbic acid as much as 150 microliters, then...

21 April 2025 5,355 6 View

To be used as a positive control, it is better to use vitamin C or vitamin E?

I conducted a cell culture study using primary fibroblast cells taken from the skin of baby mice, in the growth medium alfalfa plant extract was added. My main goal is to see the potential of...

08 April 2025 5,828 4 View

Why did my culture after 3-4 days grow mold?

I cultured primary fibroblast cells that I got from the isolation of the skin of the back of baby mice aged

07 April 2025 8,200 11 View

How do I dilute DMSO 0.1% to 0.05% in cell culture media?

I have a DMSO stock solution with a concentration of 0.1%. I want to dilute it again to a concentration of 0.05% which I will use in my cell culture media which I add with alfalfa plant extract...

03 April 2025 1,059 5 View