Tissue in liquid nitrogen can be used for pathological analysis?

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How to determine the knots when fitting the Expo-rational B-splines (ERBS) curve?

Exporational B-splines, a new type of spline function with better mathematical properties in theory (refer to [1]), require consideration during curve fitting due to the crucial impact of knots...

15 April 2024 2,129 0 View

I have a question about concatenating sequence runs in MEGA X. Can you assist me with this?

I concatenated four sequences. Can I find the best model for the concatenated sequences and then run the maximum likelihood tree with the best model in MEGA X?

14 December 2023 7,498 0 View

Why choose Phosphoric Acid/ Hydrochloric acid Acid/Sulfuric acid as the acidify agent in the extraction of short chain fatty acid?

The theory basis of this processing procedure seems unclear to me, and I have much difficulty in exploring the reason……

17 July 2023 1,894 0 View

MTT assay treat with protein sample, but it always precipitate which makes low cell viability?

As title, my sample is kind of plant-based protein(pI=4.5) and its hydrolysates and glycated protein. I treat my sample with CACO-2 cells in MTT test, but I found that my sample is ph-sensitive,...

13 May 2023 4,592 2 View

How to modify the Amber code to save the trajectory of solute only?

Dear Amber user, I want to study the interactions between protein and ions. I am running a simulation with protein, ions and water and I want to save the trajectories of protein and ions. I know...

26 March 2023 5,804 2 View

How to make Zn move along with Zn-binding domain?

Dear all Gromacs user, I am using Gromacs to run NVT and NVT equilibrium. Before the equilibrium, my input gro file shows all atoms (proteins, Zn, Na and Cl ions, and waters) stayed in the box...

17 February 2023 4,753 1 View

Can anyone suggest a good method for human lung macrophage isolation?

I am planning to isolate macrophages from human control and ARDS lungs. Can anyone suggest a good method? Thanks!

08 February 2023 4,200 0 View

How to add a zinc ion to a protein pdb file?

Dear all, I am running MD simulation of a protein containing a zinc finger (ZnF) domain. I got its all-atom pdb file and I know there are 4 sulfur atoms in 4 cycteins of ZnF domain interacting...

10 January 2023 8,701 4 View

How to run molecular dynamic simulation in 100 nsec?

Dear All, Could you tell me how to run MD simulation by any software. I have DS, VMD in hand. But I try to run 30 nsec in DS that spent 6 days, is that right? The reviewer asked me to perform...

30 October 2022 2,089 5 View

Ammonium Bicarbonate Hydrogenation to Formate in a PFR reactor converging but with no reaction happening on Aspen Plus Simulation?

Hi, I am currently conducting a simulation of CO2 conversion into Formic acid via hydrogenation of ammonia-based system. The reactants includes the aqueous ammonia and flue gas of CO2. There are...

08 October 2022 4,919 1 View

How do I replace a file with a more recent version of a paper that was uploaded to ResearchGate?

The paper in question is "Interpolation of Nitrogen Fertilizer Use in Canada from Fertilizer Use Surveys". This paper was very recently published by Agronomy (MDPI). Agronomy has, in the last day...

07 August 2024 9,934 3 View

What is the solvent for 8YSZ nano material?

I need to do some experiment with it in liquid form.

07 August 2024 613 3 View

Hi, please what is the best tool on Aspen plus that can help me get the best inlet temperature and pressure for cryogenic distillation?

I am trying to recover liquid CO2 from a mixture of 0.6 CO2, 0.3 N2, and 0.1 O2. My aim is to recover about 99% liquid CO2 at the bottom of the column and make sure the amount of gaseous CO2...

06 August 2024 4,611 0 View

How much Yeast should we add to fermentation media?

I have prepared a liquid broth containing Yeast (S. Cerevisiae) that i need to add from it to fermentation media of lignocellulosic hydrolysate. I need to know based on what parameters do we add...

06 August 2024 662 1 View

What is the best protocol for freezing the rat brain in preparation for protein detection by Elisa ?

What is the best way to freeze the rat brain - liquid nitrogen, Dry ice , isopentane - after isolation for later protein investigation by Elisa?

01 August 2024 1,722 1 View

How can we conduct DFT for solutions or mixtures ?

Can we conduct DFT studies for Ionic Liquids with Amines at various concentrations? If so, how can we proceed with these studies? Additionally, what parameters, besides IR, NMR, and HOMO-LUMO, can...

18 July 2024 319 1 View

Has anyone tried freeze flow samples in -80C freezer or liquid nitrogen before processing?

Our lab has some radioactive cell samples that need to be kept frozen for at least 30 days before flow analysis. For some reason, the samples stored in liquid nitrogen did not perform as well as...

15 July 2024 9,975 1 View

Which is true nano nitrogen or nano urea?

Some scientists use nano nitrogen and some use nano urea, even though the process used the same for preparation. So, which is true nano nitrogen or nano urea?

28 June 2024 2,885 6 View

Why does a nitrogen adsorption-desorption isotherm form two loops?

BET analysis

27 June 2024 6,964 1 View

Can a liquid metal-ligand sample be directly analyze in flame atomic absorption spectrosocopy?

Can I directly analyze a liquid metal-ligand sample without acid digestion in flame atomic absorption spectroscopy?

25 June 2024 1,866 2 View