I want to start using a multichannel pippete for preparing my pcr plates (96 wells). I know how to use the pippette propertly but I still have some doubts about the master mix preparation in terms of the reagent reservoir manipulation. how should I prepare the mastermix using this new consumable? should i just dispense the respective volumes for each pcr reagent (equivalent for the 96 wells) using a single channel micropipette? what order should i follow when adding the reagents?
i'll be very glad with the tips and information that you could share with me
thank you :)