Christian Fernando Montoya Estupiñan

6 Questions 2 Answers 0 Followers

Questions related from Christian Fernando Montoya Estupiñan

Why is it important to separate DNA and RNA extraction areas?

Why is it important to have one area for RNA extraction and another for DNA extraction. is it because of the kind of reagents that are used for RNA purification that could be more volatile and...

03 February 2022 7,744 3 View

RT-qPCR troubleshooting, what could be going wrong with this PCR?

I've been working for a long time with this probe set routinely and results in general are very good since the protocol has already been standadized. Nevertheless I have been getting this kind of...

06 April 2021 4,681 2 View

Why Im I getting this heterogenious RFU FAM fluorescence on my RT-qPCR test?

As long as I know FAM is one of the brightest used fluorophore molecule when making a qPCR test. So why Am I getting this low RFU fluorescence mixed with kind of the normal signal I would get from...

22 February 2021 6,057 1 View

What happens if i defrost the pcr buffer wrong? How is this evidenced in my pcr results?

I would like to know the consecuences of wrong reagent defrosting. does this affects reagent concentration? how do i evidence this fact on my pcr results? thank you very much for your help

27 January 2021 610 1 View

Posibilitiy of crossed contamination using same processing platafform ??

My question is related with the probability of having crossed containation when using a same processing plataform (fully automated) for viral load diagnosis of different kind of viruses. Ex: can i...

30 November 2020 5,241 3 View

RT-qPCR troubleshooting. What could be generating this kind of result?

I dont know why im getting this kind of amplification curves. Anyone has an opinion respect to this issue ?

28 September 2020 8,482 5 View