8 Questions 2 Answers 0 Followers
Questions related from Christian Fernando Montoya Estupiñan
Why is it important to have one area for RNA extraction and another for DNA extraction. is it because of the kind of reagents that are used for RNA purification that could be more volatile and...
03 February 2022 7,751 3 View
Does anyone knows why could this be happening? I got a positive result for my qualitative q-PCR test detection for Cytomegalovirus, but when I tryed to quantify the viral load associated with this...
01 February 2022 1,142 1 View
I've been working for a long time with this probe set routinely and results in general are very good since the protocol has already been standadized. Nevertheless I have been getting this kind of...
06 April 2021 4,686 2 View
As long as I know FAM is one of the brightest used fluorophore molecule when making a qPCR test. So why Am I getting this low RFU fluorescence mixed with kind of the normal signal I would get from...
22 February 2021 6,070 1 View
I would like to know the consecuences of wrong reagent defrosting. does this affects reagent concentration? how do i evidence this fact on my pcr results? thank you very much for your help
27 January 2021 624 1 View
I want to start using a multichannel pippete for preparing my pcr plates (96 wells). I know how to use the pippette propertly but I still have some doubts about the master mix preparation in terms...
22 December 2020 2,777 0 View
My question is related with the probability of having crossed containation when using a same processing plataform (fully automated) for viral load diagnosis of different kind of viruses. Ex: can i...
30 November 2020 5,245 3 View
I dont know why im getting this kind of amplification curves. Anyone has an opinion respect to this issue ?
28 September 2020 8,496 5 View
