Which anesthetic for cortisol determination in fish?

I am a bit confused. I am planning an experiment in the field where fish will be caught by electrofishing. Afterwards, they are supposed to be euthanised by using an anaesthetic. I found...

10 February 2021 1,101 4 View

What is the first thing that comes to your mind when you think of the effectiveness of foreign aid on development? Effective/not effective, why?

I'm currently doing a research project on the effectiveness of U.S. aid in Georgia, and would very much appreciate your insights.

22 December 2020 4,189 1 View

Can peptides survive high temperatures?

I'm looking for ways to functionalise a material with a cell-adhesive peptide (relatively short chain - 6aa) and I was just wondering if peptides can withstand high temperatures (100-150 C) for 30...

15 January 2020 8,978 4 View

Abnormally high fluorescence read with qpcr, and no amplification showing on agarose gel, what could be going on?

Hi! I've been running qPCR on some drinking water samples with a primer/ probe FAM assay. In any given run, there have been at least a few samples with artificially high fluorescence reads,...

16 October 2019 8,060 3 View

How to prepare agar amended with mercuric chloride?

Several studies mention using agar amended with mercuric chloride when isolating mercury resistant bacteria. Is there a lab method available for preparation of these plates? If no, what are some...

17 June 2019 5,047 5 View

Interview guides for UTAUT?

30 January 2019 5,443 4 View

How much does our childhood affect the way we think and make investment choices?

Subconscious, subjective drivers.

22 January 2019 5,397 5 View

ReNcell VM cell culture problems - why are they dying after I split them?

The cells look healthy after thawing, but they die when I try to split them. I have tried making fresh media and fresh matrigel, and have tried filtered and non-filtered Accutase. Does anyone have...

19 December 2018 630 9 View

Is it necessary to dechorionate zebrafish embryos to extract genomic DNA and RNA ?

I tried to dechorionate embryos with pronase at 1mg/mL. I made 3 washes but I obtained a high mortality rate and the medium was dirty. Thank you for your answers

03 January 2017 3,455 4 View

Can you help me with Activity measure of trypsin?

Sustrate=BAPNA I get a white solution when i mixed BAPNA(with DMSO) and buffer Tris-HCL/CaCl2 First 3 minutes the activity goes down, after 3 min stars to go up but slowly. Is this normal?

25 July 2016 6,718 7 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

Image Processing and classification using keras/Tensorflow?

Do you have some recommendations approaches on Image processing and classification of plant diseases using TensorFlow/Keras?

02 March 2021 1,493 4 View

Which SAR dataset to use for generating coherency matrix and performing target decomposition (Description) ?

Working on chandrayaan-2 DFSAR data, there are three datasets available: 1) Slant range image data product: The slant range complex image file. Each pixel is represented by two 4-byte floating...

02 March 2021 8,481 3 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View