Please I want to know the method for measuring bile salts in bird how I do this in lab please help me to know the method?

I need to the required sample ,reagents and steps

10 December 2020 7,087 1 View

I need to know the approach to solve this task.. How high the permeate-specific operating cost in Nano filtration process??

The total pressure loss = 1 bar Pump efficiency : 25%.. I need to know the approach, the work flow, the equation needed please. Thanks in advance

14 March 2020 9,783 3 View

What is the main drug for treatment of oral mycosis ?

oral mycosis infection

11 September 2019 6,746 3 View

Thermophilic gram- negative bacteria and spore forming which one ? name the bacteria or genus of bacteria ?

more information about thermophilic bacteria

11 August 2019 6,750 1 View

Please, i want to learn about UV graph ?

the blue circle in uv graph can consider as the head of graph and can i take this points the value of the wavelength of nanoparticles ? in the photo with number 1 this graph indicates that there...

09 August 2019 158 1 View

Hello, dear researchers. I'd like ask about your opinion about this UV graph?

first image for SNPs synthesized by certain plant, while second image is the UV of plant extract without silver nitrate i think the wavelength of SNPs start from 300 to 450 nm? thank you

06 August 2019 5,311 2 View

Is there any method or software to convert 3 equation with 4 unknown parameters into one equation?

Solve equations

15 May 2019 5,924 4 View

What is the best and fastest method of removing solvent like THF from Tecothane (polymer) to form a homogeneous layer?

I want to remove THF from my polymer (Tecothan) . If I use the vacuum oven ,it will affect the homgeneity of the formed layer?? Or not?? Is there any solution for this problem?

15 November 2018 3,825 2 View

How can i preserve cell culture media for long time?

I have two bottles of cell culture media MEM Hanks and 199 Earls I dont need them now, but i will later. How can I preserve them for about 6 months , without loss in nutrients or contamination ?

24 May 2018 3,138 3 View

The appropriate solvent for Tecothane

02 August 2017 1,391 2 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

How to regulate SSCP temperature in simple PAGE?

I'd like to perform single-strand conformation polymorphism (SSCP) in my thesis, however I cannot control the temperature of the vertical PAGE since we are using the conventional tanks. Is there a...

02 March 2021 9,157 1 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

How to compare 2 samples recruited with different sampling methods?

If I want to compare characteristics of 2 groups of patients, one group is recruited from a random sample of the general public while another group is from a sample with a fixed gender (male to...

26 February 2021 3,526 3 View

How can I assign a velocity along one direction to CO2 molecules in a molecular dynamics simulation using GROMACS?

Dear all, I am trying to perform a MD simulation about the interaction between CO2 and a polymer. I create my model, solvate it and perform the NVT and NPT equilibration. Now I am not able to...

26 February 2021 10,014 3 View

LEDs on Vis Light photo degradation of MB water sol via N doped TiO2 in a mini flume?

Good day to all, I have no experience with light emitting diodes. I am building a glass mini flume to demonstrate the photo degradation of Methylene Blue in water running over self cleaning...

26 February 2021 4,026 5 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View