I am trying to define the threshod of COVID 19, E GENE detection using ABI 7500. WE are using KOREAN KIT powercheck however, the THRESHOLD is not define in the package insert, the FLUOROPHORE is FAM.
Hi Ali Bazzi , has this been resolved for you guys there.
Also are you detecting another gene of interest as the E-gene is nonspecific to Covid19. Something like ORF or the N2 gene would be needed to have more specificity. I would also get in touch with the manufacturer for guidence