hi everyone 

this is the second time  i am asking this urgent question What are the reasons of jagged qRT-PCR fluorescence and melting curves ?

i am working with human SNP  resulting in two primer fragments please i need to know the reasons of jagged fluorescence and melting curves appeared occasionally during qPCR process , is that related to technical error, handling, software or instrumental problems,  or something else ???

i am attaching some photos of my recent results 

 i will appreciate your help 

thank you

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