On some E coli cells, I see some opening of cell wall as shown in this image. Please guide what is it and why such things happen i.e. under what kind of conditions ?
It could be damage made during cell preparation for electron microscopy.
Of course they're "dead." This is SEM.
They are dead bacteria (destroyed during preparaition for electron microscopy smear ).
Dead cells, as cell preparation is for electron microscopy
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