Thinking about starting some experiments using chemostat. Is this something I can buy, or do we need to build using components?

More A. Malcolm Campbell's questions See All

How can I remove oil from droplets formed by microfluidics?

I want to move into using nano-scale aqueous droplets to conduct biochemical reactions. Anyone have good ways to remove the oil after reaction is completed?

03 April 2019 4,734 3 View

Do you have a commercial source for outsourcing DNA sequencing?

I want to send out a 96 well plate containing bacterial cultures and have all 96 mini-prepped and sequenced by the company. Does anyone know of a commercial source for this and possible pricing?

02 March 2014 354 8 View

We have a plasmid that contains kanamycin resistance but it allows E. coli to grow on ampicillin as well as kanamycin.

Have others seen this too? Any suggestions how to fix it?

01 February 2013 428 17 View

No title

02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

Giuseppe Inesi

The re is a very nice and recent writeup on chemostat by wiekepedia, with severa l specific references. I am sure that they will be helpful

Mary Lee Stewart Ledbetter

How precisely do you need conditions to be controlled? And what volume? A relatively simple flow-through system would involve inflow and outflow ports with aeration, circulation, and heating of the contents. A 1L or larger filter flask might do the job...